Journal: Cell Reports Medicine

Article Title: Delayed viral rebound post-ART interruption in infant macaques given SIV-specific neutralizing antibodies

doi: 10.1016/j.xcrm.2026.102636

Figure Lengend Snippet: SIV-specific T cell responses and correlation with viral rebound AUC (A) SIVgag-specific CD8 + T cell responses were quantified by AIM assay, using Boolean gating to show the frequencies of CD69 + CD25 + , CD69 + OX40 + , or CD25 + OX40 + cells following stimulation with an SIVgag peptide pool after background subtraction. The pre-ATI (and pre-RhmAb for that group) time point was compared to the rebound peak post-ATI. Nonparametric Wilcoxon matched-pairs signed rank test was used for statistics. N = 4 and N = 5 for controls at each time point based on sample availability. (B) Graph shows the frequency of SIVgag-specific CD8 + T cells at peak rebound in the RhmAb group and rebound viral load AUC. Statistical analysis was performed by calculating the nonparametric Spearman’s rank correlation coefficient (r) that was compared to zero.

Article Snippet: PE-Vio770 Mouse Anti-Human CD25 , Miltenyi Biotec , 130-113-287; RRID:AB_2784092.

Techniques:

Journal: Journal of Cancer Research and Clinical Oncology

Article Title: Regulatory T cells inhibit FoxP3 to increase the population of tumor initiating cells in hepatocellular carcinoma

doi: 10.1007/s00432-024-05892-2

Figure Lengend Snippet: ‘Stemness’ of HCC-LM3 cells was enhanced after co-cultured with Tregs. ( a ) Isolation and expansion of CD4 + CD25 + CD127- Tregs. ( b ) Flow cytometry analysis showing high expression of CD4, CD25, and FoxP3 in Tregs. ( c ) Co-culture of encapsulated HCC cells and Tregs. ( d ) Immunofluorescence staining of CD133 in HCC cells before and after co-culture, and ( e ) HCC TIC (CD133 positive cells) ratio before and after co-culture, student’s t-test, * P < 0.05, compared with the control group. ( f ) RT-qPCR analysis of HCC TICs-related genes and ( g ) EMT-related genes, n = 3, student’s t-test, * P < 0.05, compared with the control group. ( h ) Western blot analysis of β-catenin and c-Myc in HCC cells before and after co-culture

Article Snippet: Tregs were labeled with FITC Mouse Anti-Human CD4 (1:5) (561,005, BD Biosciences, Franklin Lakes, NJ, USA), PE Mouse Anti-Human CD25 (1:5) (555,432, BD Biosciences) and Alexa Fluor ® 647 Mouse anti-Human FoxP3 (1:20) (561,184, BD Biosciences) antibodies for 30 min on ice, followed by washing with phosphate buffered saline (PBS) (Gibco), FITC Mouse IgG1 (555,748, BD Biosciences), PE Mouse IgG1 (555,749, BD Biosciences), Alexa Fluor ® 647 Mouse IgG1 (557,732, BD Biosciences) were used as isotype controls.

Techniques: Cell Culture, Isolation, Flow Cytometry, Expressing, Co-Culture Assay, Immunofluorescence, Staining, Control, Quantitative RT-PCR, Western Blot